ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2017, Vol. 48 ›› Issue (11): 2084-2090.doi: 10.11843/j.issn.0366-6964.2017.11.009

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Effects of Insulin and FSH on E2 Production of Pig Ovarian Follicular Granulosa Cells in vitro Culture

LI Peng-fei1, MENG Jin-zhu2, HAO Qing-ling1, BI Xi-lin3, WANG Kai3, ZHU Zhi-wei1, LÜ Li-hua3*   

  1. 1. College of Life Science, Shanxi Agricultural University, Taigu 030801, China;
    2. Wujiang College, Tongren University, Tongren 554300, China;
    3. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
  • Received:2017-06-15 Online:2017-11-23 Published:2017-11-23

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Abstract:

To research the effects of insulin and FSH on porcine ovarian follicular development, ovaries were collected and follicular granulosa cells (GCs) were isolated. Setting control groups and repetitive groups, GCs were cultured for 7 d treated with different concentrations of insulin and FSH by long-term culture in vitro. Observing the growth situation and counting the numbers of GCs in each well. Competition method was performed to detect the E2 concentrations in medium. Results showed that, at 0 ng·mL-1 concentration of FSH in medium, with the increase of insulin concentration, GCs proliferation was more and the number of GCs showed a tendency to increase. The numbers of GCs were the most when the supplement dose of insulin was 100 ng·mL-1 (P<0.05). When insulin was 0 ng·mL-1, with the increase of FSH concentration, GCs proliferation was more and the number of GCs showed a tendency to increase, and at 5 or 25 ng·mL-1 concentration of FSH, the number of GCs were more than other 2 groups (P<0.05). When FSH at 0 ng·mL-1, insulin at 100 ng·mL-1, E2 concentration was more than other groups with different concentration of insulin (P<0.05). When insulin was 10 ng·mL-1 and FSH was 1 ng·mL-1, E2 concentration in the medium was the greatest, but there was no significant differences among groups with different concentrations of insulin (P<0.05). At 5 or 25 ng·mL-1 concentration of FSH, there was no significant differences in E2 concentration among different groups with different concentrations of insulin. Insulin in cooperation with FSH promotes the proliferation and E2 production of porcine follicular GCs. The E2 secretion of porcine follicular GCs will decline when FSH exceeded a certain physiological concentration.

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